Antibiotic Resistance against Escherichia coli and Salmonella sp. Isolates in Chicken Meat and Cloacal Swabs in Sidoarjo District
Keywords:
antimicrobial resistance , Escherichia coli , food-borne disease, multidrug resistance, Salmonella sp.Abstract
Background: Antimicrobial resistance is a global health problem and crisis, complicating the treatment of foodborne diseases caused by pathogenic bacteria, such as Escherichia coli or Salmonella sp.
Objective: This research aimed to determine the presence of E. coli and Salmonella sp. bacterial contamination and to assess antibiotic resistance against these pathogens in chicken meat samples and cloacal swabs in Sidoarjo District, East Java Province.
Method: A total of 67 samples of minced chicken meat and 50 samples of cloacal swabs were collected from several sub-districts in Sidoarjo District. The samples were isolated and identified using MacConkey Agar (MCA) media for E. coli and Salmonella Shigella Agar (SSA) for Salmonella sp., followed by Gram staining, biochemical tests, and carrying out resistance tests on Mueller Hinton Agar (MHA) media.
Results: The study identified E. coli contamination in 94% of samples and Salmonella sp. in 14%. The highest resistance against E. coli from chicken meat samples was to amoxicillin (69%, 43/62) and ampicillin (68%, 42/62) and from cloacal swabs samples was to tetracycline (52%, 25/48). For Salmonella sp., the highest resistance in chicken meat and cloacal swabs was to tetracycline (75%, 9/12 and 80%, 4/5 for chicken meat and cloacal swabs, respectively).
Conclusion: The study revealed a high contamination of E. coli and Salmonella sp. bacteria in chicken meat and cloacal swabs, with both bacteria showing high resistance to amoxycillin, ampicillin, and tetracycline.
