Polymorphism and Association of BGN Gene with Cholesterol in Lamb Meat

Abstract

The cholesterol levels in meat serve as an assessment for individuals selecting healthy meat options, correlating with an increased understanding of its importance to health. The Biglycan (BGN) gene could impact cholesterol levels in lamb meat. This study aims to find variants in the BGN gene as potential genetic determinants of cholesterol levels in lambs. A total of 87 rams from Indonesia were utilized as research samples. This study employed the PCR-RFLP technique and showed that the BGN gene exhibited polymorphism at the HinfI restriction site, providing two genotypes (GG and CC). The correlation between BGN genotypes and lamb meat cholesterol was examined utilizing the General Linear Model (GLM). The findings of this research found a significant association (p < 0.05) between the BGN gene and cholesterol levels in lamb meat. The GG genotype shows reduced cholesterol levels in comparison to CC. These findings can improve knowledge of the BGN gene's contribution, with the SNP point g. 77807325 G>C identified as a potential candidate for cholesterol reduction in lambs.