In vivo efficacy of supernatant based cell microbial consortium in managing fusarium wilt of banana

Abstract

. Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense, is a major osbtacle of banana production worldwide. Conventional chemical and cultural control measures often associated with the environmental damage and fail to provide sustainable solutions. Exploring biological alternatives is considered a future approach. This study was to investigate the in vivo efficacy of a supernatant-based cell microbial consortium (SBS) in suppressing Fusarium wilt in banana seedlings. The experiment was arranged using Randomized block design with 9 treatments; 1). water (control treatment), 2). SBS BurkholderiaS10KTR316, 3) SBS Burkholderia S10KTR325, 4) SBS TrichodermaPP21, 5). SBS TrichodermaKPK22, 6) SBS TrichodermaPP21+SBS BurkolderiaS10KTR316, 7). SBS TrichodermaPP21+ SBS BurkolderiaS10KTR325, 8). SBS TrichodermaKPK22 + SBS BurkolderiaS10KTR316, 9) SBS TrichodermaKPK22 + SBS BurkolderiaS10KTR325. Each treatment was replicated four times and five seedlings as the experimental unit. Greenhouse experiments were conducted by inoculating one-month old banana seedling with either as monoculture or combined SBS microbial at planting time. Application of Foc inoculum was at 15 days after planting. Disease severity, disease incidence, and plant growth parameters were recorded over the two months. The results revealed that single and co-cultivation treatment of SBS microbial were not significantly reduced percentage of wilt symptoms, disease incidence and severity compared to untreated controls. SBS BurkholderiaS10KTR325 increased plant height, stem circumference, and leaf number compared to the control. Field-scale evaluation is considered to confirm consistency under diverse agro-ecological conditions.