Enhancing Total Phenolic, Flavonoid, and Antioxidant Capacity in Black Orchid (Coelogyne pandurata Lindl.) with Paclobutrazol Application

Abstract

Black Orchid (Coelogyne pandurata Lindl.) is an orchid species with potential secondary metabolite content, including total phenolic content, total flavonoids content, and antioxidants, holds significant potential in horticultural applications. This study aimed to evaluate the effect of Paclobutrazol spraying on the total phenolic content, total flavonoid content, and antioxidant capacity in Black Orchid (C. pandurata). The experimental design used in this study was a randomized block design (RCBD) with Paclobutrazol treatments at concentrations of 0, 50, 100, and 150 ppm. Each experimental unit was conducted with three replicates. Total Phenolic, flavonoid, and antioxidant capacities were analyzed using a Nano-spectrophotometer. Results indicated that treatment with 150 ppm Paclobutrazol significantly increased the flavonoid concentration in the leaves, although no notable effect was observed on the total phenolic content or antioxidant activity in both leaves and bulbs.