In Vitro Study on Alpha Amylase Inhibitory Activity of a Breadfruit Peel Extracts (Artocarpus Altilis)

This paper was not presented at the conference.

Abstract

This paper was not presented at the conference.

The objective of this study was to evaluate the alpha-amylase inhibitory activity of different extracts of Artocarpus altilis against human salivary amylase in vitro. The plant extracts were prepared sequentially with n-hexane, ethyl acetate and methanol. Each extract was evaporated using rotary evaporator, under reduced pressure. The thick extracts was analyzed using gas chromatography mass-spectroscopy (GCMS) and four transform infra-red (FTIR). Different concentration (10, 5, 3, 1 mg/mL) of each extract were made by using buffer phosphate pH 6,8 and subjected to -amylase inhibitory assay using starch as a substrate and dinitrosalysilic acid method. The absorbance was read at 502 nm using spectrophotometer. Using this method, the n-hexane, ethyl acetate, methanol extracts and acarbose exhibited alpha-amylase inhibitory activity with an IC₅₀ values 51,21 mg/mL, 38,71 mg/mL, 57,44 mg/mL and 49,69 mg/mL respectively. GCMS results of each extracts showed that n-hexane extract had 71 peak where squalene has a higher area (16,75%), while ethyl acetate extract had 88 peak and 1-(1,5-dimethyl-4-hexenyl)-3a,6,6,12a-tetramethyltetradecahydro- has a higher area (19,22%). And methanol extract had 80 peak with hexadecenoic acid shown a higher area (18,085%). Each extract was analyzed using FTIR and showed that each extract contained alkaloid, flavonoid and terpenoid/steroid. Ethyl acetate extract showed greater alpha-amylase inhibitory activity than acarbose. Ethyl acetate extract showed greater alpha-amylase inhibitory activity than acarbose.