In Vitro Studies on the Inhibition of α-Amylase by Extract of Sambiloto (Andrographis Paniculata) Stem

This paper was not presented at the conference.

Abstract

This paper was not presented at the conference.

Diabetes mellitus is a metabolic disease characterized by hyperglycemia. Sambiloto is a traditional medicine that is frequently used by the Indonesian populace. The aim of this study was to conduct the anti-diabetic activity of the hexane, ethyl acetate and methanol extract of sambiloto stem by in vitro. The Sambiloto stem extracts were prepares sequentially with hexane, ethyl acetate and methanol then analyzed using GC MS and FTIR. The inhibitor activity test of the α-amylase enzymes is carried out using the DNS method by spectrophotometry. The hexane, ethyl acetate and methanol extract of sambiloto stem had α-amylase enzyme inhibitory with IC₅₀ values of 38,33; 38,31; and 28,21 mg/mL, respectively. Based on the GC MS results, 87 possible compounds detected in the n-hexane extract with the highest peak was 9,12-Octadecadienoic acid. The results of the GC MS ethyl acetate extract yielded 73 compounds with the original composition of 1-(1,5-Dimethyl-4-Hexenyl)-3a,6,6,12a-Tetramethyltetradecahyd and 89 compounds detected in the methanol extract with the highest peak was 5-Hydroxy-3',4',6,7-tetramethoxyflavanone. Based on the research results, it can be concluded that hexane, ethyl acetate and methanol extract from sambiloto stem have anti-diabetic property, among these three extract, ethyl acetate showed potent enzyme inhibition as compared to other extracts.