Utilization of whey-based growth media for production of recombinant papain-like protease of SARS- CoV-2 under Escherichia coli host cells

This title has been presented on Friday, December 15, 2023 at 13.30-13.40 GMT+7.

Abstract

This title has been presented on Friday, December 15, 2023 at 13.30-13.40 GMT+7.

Papain-Like Protease (PLpro) in the SARS-CoV-2 virus is considered a viable target for drug development to treat Coronavirus Disease 2019 (COVID-19) patients. The commonly used method for PLpro production is through a recombinant approach using Escherichia coli as a host cell, which is simple and affordable. However, this still requires expensive growth media (Luria Bertani/LB) for host cell cultivation. This study aims to investigate the possibility of using whey as an alternative growth medium for producing PLpro from SARS-CoV-2. A combination of whey and LB media using different concentrations of whey (0%, 25%, 50%, and 100%) was tested. Cell density in each medium was monitored using a UV-Vis spectrophotometer and PLpro expression was induced by adding 1 mM IPTG, and analyzed using SDS-PAGE. Data were analyzed using descriptive analysis. The results showed that E. coli host cells for PLpro production from SARS-CoV-2 can be successfully recombinant using whey media. The best treatment was found in the medium composition of 50% whey.