Extraction of virgin deep-sea fish oil (Lepidocybium flavobrunneum and Ruvettus pretiosus) and their potential as immunostimulants

This title has been presented on Thursday, December 14, 2023 at 16.10-16.20 GMT+7.

Abstract

This title has been presented on Thursday, December 14, 2023 at 16.10-16.20 GMT+7.

Escolar (Lepidocybium flavobrunneum) and oilfish (Ruvettus pretiosus) are by-catches of tuna and classified as deep-sea fish which can live at a depth of 200 meters below sea level. These fish can be used as a source of making fish oil due to their high fat content up to 22%. Based on the previous research, deep-sea fish have potential as source of food and medicine. Therefore, this research aims to determine fat content, oil yield, fatty acid profile, oxidation parameters, and the potential of deep-sea fish oil as an immunostimulant agent by in vitro method. Deep-sea fish oil extraction was carried out using the low-temperature wet rendering (40, 50, 60, and 70 °C) method and determining splenocyte proliferation using the MTT assay. The dominant fatty acid in escolar and oilfish were oleic acid (C18:1n-9c) 26,85% (w/w) and 23,02% (w/w), respectively. Splenocyte proliferation activity showed that oilfish and escolar fish oil could increase splenocyte proliferation, so they had the potential to act as an immunostimulant.