Ligation of Femoral Artery Induced Systemic Ischemia Re-perfusion Injury Which was Attenuated by Ultrasound of the Vagal Nerve

This title has been presented on Thursday, December 14, 2023 at 13.20-13.30 GMT+7.

Abstract

This title has been presented on Thursday, December 14, 2023 at 13.20-13.30 GMT+7.

Ischemia reperfusion injury (IRI) is an injury caused by a biochemical reaction 
arising from reperfusion after a period of ischemia. This study was conducted to evaluate the histological damage of various organs, intestinal motility, CK-MB, and LDH levels after IRI and ultrasound (US) therapy on the vagus nerve. The study was carried out on 24 Sprague-Dawley rats which were divided into 4 groups namely naive (N), control (C), no treatment (NTR), and treatment (L). Ischemia was induced by femoral artery ligation for 6 hours and followed by reperfusion for 30 minutes. Therapy was given to group L with an intensity of 1,5 W/cm 2 . Based on histopathological observations, the lungs, liver 
and small intestine of the groupd affected by IRI experienced mild pathological changes. No  abnormalities  were  found  in  the  heart  muscle,  spleen,  and  kidney.  The histopathological data of group L indicated that the therapy did not exacerbate tissue damage. In the IRI-affected groups, the intestinal motility were decreased significantly. CK-MB  and  LDH  levels  tend  to  increase  which  indicates  tissue  damage.  Ultrasound therapy during reperfusion was able to attenuate intestinal motility, CK-MB, and LDH 
levels  close  to  normal  values.  The  arterial  ligation  method  used  resulted  in  a  mild ischemic degree, therefore resulting mild reperfusion injury. Ultrasound therapy has a tendency to repair tissue damage caused by IRI.