Phenotypic and Genotypic Identification to S. aureus isolates from Cattle in Bogor

This title has been presented on Thursday, December 14, 2023 at 15.40-15.50 GMT+7.

Authors

  • Sri Suryatmiati Prihandani National Research and Innovation Agency Author https://orcid.org/0000-0001-5572-6325
  • Susan Maphilindawati Noor National Research and Innovation Agency Author
  • I Wayan Teguh WIbawan School of Veterinary and Biomedical, IPB University Author
  • Sumirah Sumirah National Research and Innovation Agency Author
  • Safika Safika School of Veterinary and Biomedical, IPB University Author
  • Bagus Nugraha Darmayanto Transfarma Medica Indah Author

Keywords:

Genotypic identification, PCR, Virulence factor, Staphylococcus aureus

Abstract

This title has been presented on Thursday, December 14, 2023 at 15.40-15.50 GMT+7.

The bacterium Staphylococcus aureus, sometimes known as S. aureus, is responsible for numerous infectious disorders that affect both human and animal health. The purpose of this work is to phenotypically and genotypically identify S. aureus from previously obtained isolates that were originally from cattle. Phenotypic identification of Staphylococcus aureus was determined based on biochemical tests including Gram staining, triple sugar iron agar, catalase, and coagulase tests. Genotypic identification was determined based on detection of the presence of 23SrRNA and nuc genes using specific primers for Staphylococcus aureus by polymerase chain reaction (PCR). In this activity, standard Polymerase Chain Reaction (PCR) for genotypic detection is focused on targeting the nuc genes as virulence factor. There were seven isolates in total are positively detected for having the nuc genes which indicated has virulence factor of S. aureus strain.

Author Biographies

  • Sri Suryatmiati Prihandani, National Research and Innovation Agency

    livestock health and veterinary public health research groups of Veterinary Research Center

  • Susan Maphilindawati Noor, National Research and Innovation Agency

    livestock health and veterinary public health research groups of Veterinary Research Center

  • I Wayan Teguh WIbawan, School of Veterinary and Biomedical, IPB University

    Veterinary; Veterinary science, Immunology and Immunopatology

  • Safika Safika, School of Veterinary and Biomedical, IPB University

    veterinary science, microbiology, parasitology, biochemical science

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Published

2023-11-30

Issue

2023: 2023 Program Book FiSAED: Environmental Health and Ecosystems

Section

Environmental Health and Ecosystems

Categories