Automation of bull semen analysis through the development of a modular open technology device for cryopreservation

This title has been presented on Friday, December 15, 2023 at 13.45-13.55 GMT+7.

Abstract

This title has been presented on Friday, December 15, 2023 at 13.45-13.55 GMT+7.

Cattle semen quality analysis is an important step before processing it into frozen semen for artificial insemination. Transportation from the shelter to the laboratory can reduce semen quality and even kill spermatozoa. This research aims to develop a prototype that can analyse cattle semen quality automatically  using a modular open-technology device with multi-digital sensors that is comparable with standard laboratory measurements. The prototype components consisted of the following: 1) input: syringe as semen container to be tested, DIY water level sensor for volume measurement, TDS sensor for viscosity measurement, pH sensor for pH value measurement, and LDR sensor for colour measurement; 2) process: Arduino Mega 2560 as a micro-controller; and 3) output: LCD 16x2 for displaying the measurement. The results showed that the coefficient of determination for the volume, TDS, and pH sensors were 1, 0.9993, and 0.9987, respectively. Data from the TDS and LDR sensor calibration results show three viscosity levels (low: 300-599 ppm, moderate: 600-999 ppm, and high: 1.000-1.400 ppm) and three colour quality values (cloudy white: 40-59 Ω, milky white: 60-89 Ω, and creamy white: 90-130 Ω). The device developed was able to analyse the semen quality in five seconds and displayed on the LCD screen.