The addition of probiotic in selected production media and enzymes on rumen fermentability characteristics

This title will be presented on Friday, December 15, 2023 at 10.15-10.25 GMT+7.

Abstract

This title will be presented on Friday, December 15, 2023 at 10.15-10.25 GMT+7.

This study aims to evaluate the addition of probiotics Lactiplantibacillus plantarum (P), combination enzymes (CE), and enzyme stimulation secretion (ESS40) to feed in vitro fermentation system. The study was conducted in two stages, probiotic level testing using a completely randomized design with 3 treatments (P1: 2%; P2: 5%; P3: 10%), fermentative testing using a randomized block design with 12 treatments (X0 = basal ration; X1 = X0 + 1% ESS40; X2 = X0 + 0.5% CE; X3 = X0 + 1% CE; X4 = X0 + 1% P; X5 = X1 + 0. 5% CE; X6 = X1+ 1% CE; X7 = X1 + 1% P; X8 = X2 + 1% P; X9 = X3 + 1% P; X10 = X5 + 1% P; X11 = X6 + 1% P). Data was analyzed using analysis of variance (ANOVA) and significant results (P<0,05) were tested using Duncan's test and orthogonal contrast. Parameters was probiotics microbiology, pH, concentration of NH3, IVDMD, IVOMD, VFA, total gas, and methane production. The use of inoculant levels had a significant effect P<0.05) on pH and total lactic acid content. The addition of 10% inoculant had an effect on increasing the population and concentration of lactic acid in the probiotic production media. The addition of probiotics, CE and ESS40 had a significant effect on the VFA, total gas and methane production. The conclusion of this study is that the use of probiotics, CE, and ESS40 had not significantly affected rumen fermentation products, but significant to reduce methane production in the rumen