Antibacterial activity assay of marine fungal extract Aspergillus terreus (WB 1-2) against Escherichia coli cause of Colibacillosis disease

This title will be presented on Friday, December 15, 2023 at 10.25-10.35 GMT+7.

Abstract

This title will be presented on Friday, December 15, 2023 at 10.25-10.35 GMT+7.

Colibacillosis is one of the infectious diseases of ruminants and poultry caused by Escherichia coli bacteria. Marine fungi have bioactive antibacterial compounds. The aim of this research was to assess the antibacterial activity of extracts of endophytic marine fungal isolates associated with mangrove Aspergillus terreus (WB 1-2) to inhibit the growth of pathogenic bacteria E. coli. The research was carried out with several stages of cultivation of fungal, extraction of active compounds, and in vitro test of antibacterial activity. The extraction was performed by maceration method using ethyl acetate solvent and concentrated with rotary evaporator. Testing of antibacterial activity using the paper disc diffusion method. The measured parameter is the magnitude of the inhibitory diameter formed around the disc paper. Variations in the concentration of the sample used were 0.5; 1 and 2 mg. In this research, ethyl acetate (sample solvent) was used as a negative control and Chloramphenicol as a positive control. The results showed that extract of Aspergillus terreus (WB 1-2) showed the highest inhibition zone at a 2 mg/µL concentration of 17.60±5.35 mm was categorised as strong and had bacteriocidal activity.