Detection of genetic markers in the zoometrical indices of Indonesian local cattle with Bovine 50K SNP BeadChip

This title will be presented on Friday, December 15, 2023 at 14.15-14.25 GMT+7.

Abstract

This title will be presented on Friday, December 15, 2023 at 14.15-14.25 GMT+7.

Body conformation is one of the selection methods in livestock animals that is assessed with body measurements. The present study aimed to determine the genetic markers in zoometrical indices of Indonesian local cattle. Fourty-five (45) heads of cattle consisting of Bali (16 heads), Madura (16 heads) and Peranakan Ongole / PO (13 heads) were collected from the breeding station for the experimental animals in this study. A Bovine 50K SNP BeadChip  (Illumina, USA) was used to explore genetic mutation in the autosomal chromosomes. Nine (9) zoometrical index parameters were evaluated in the present study. A TASSEL 5.0 software were used for detecting mutation sites with General Linear Method (GLM). Research showed that two SNP markers of ARS-BFGL-NGS-115374 (SNP1) at BTA14 and Hapmap28985-BTA-73836 (SNP2) at BTA5 had a significant association with zoometrical indices in pool animals based on Manhattan plot . The SNP1 had a significant association with the area index (4.38E-6). Subsequently, a SNP2 significantly correlated with body ratio (6.47E-6) and over-increase index (1.85E-6) parameters. In addition, SNP1 is not located  in the gene region. Meanwhile, SNP2 is located at the intron 5 region of the Cryptochromes circadian regulator 1 (SRY1) gene. However, both SNPs are monomorphic in Bali  cattle  (Bos javanicus) but polymorphic in Bos indicus cattle (Madura and PO). In conclusion, the CRY1 gene can improve the zoometrical indices of Bos indicus cattle of Madura and PO. Further studies to investigate the CRY gene with large samples are essential for evaluating this gene accurately