Profile of Bromelain Enzyme Extracts by Different Precipitation Methods and Effect of pH and Temperature on Protease Activity

Abstract

Bromelain is a proteolytic enzyme in pineapple that can be processed into a feed additive. This research used pineapple peel to obtain bromelain through extraction and precipitation from Kediri district, East Java, Indonesia, by applying three treatments: crude extract, precipitation of bromelain with ethanol, and ammonium sulfate. The results showed significant differences in total protein and enzyme activity in all treatments. Duncan's multiple range test on total protein showed that treatment with crude extract (T0) and 90% ethanol (T1) had no significant effect. However, treatments T0 and T1 have a significant impact compared to other treatments. The results of the enzyme activity test showed that the T1 treatment had the highest enzyme activity value. The results of Duncan's multiple range test showed that the enzyme activity of treatments T0, T1, 80% ethanol (T2), and 60% ammonium sulfate (T8) did not have a significant effect. However, compared with other treatments, this treatment has a significant effect.   The crude enzyme extract has greater potential to be used as a feed additive because it is more efficient, affordable and has almost the same quality as 80%, 90% ethanol and 60% ammonium sulfate treatment in terms of total protein and enzyme activity.