ANALYSIS OF SINGLE NUCLEOTIDE POLYMORPHISMS (SNPs) ACCESSIONS OF SENGON (Falcataria falcata) TOLERANT AND INTOLERANT TO BOKTOR (Xystrocera festiva) PESTS

Abstract

Sengon is one of the forest trees that always be susceptible to pest and disease attacks, especially attacks from Boktor pests. This research aims to evaluate the growth of sengon progeny test grown in the Kediri Forest Management Unit (FMU), Perum Perhutani, and analyze the relationship between Single Nucleotide Polymorphisms (SNP) markers and their phenotypes of tolerant and intolerant against Boktor pests. The samples consisted of tolerant accessions (R28, R29, R30, R33, and R47) and intolerant ones (S13, S21, S22, S24, and S25), with 4 individuals replication for each accession. All samples were genotyped using 4 SNP primers applying the High-resolution Melting (HRM) at the Forest Genetics Laboratory and Advanced Research Laboratory of IPB University. Data were analyzed using the Mann-Whitney test, chi-square test, and visualized in a Manhattan plot, and Principal Component Analysis (PCA). The phenotype assessment showed a high level of pest and disease attacks in both tolerant and intolerant accessions at 84.33% and 79.64% respectively, with no significant difference between the two Sengon accessions. The application of SNP primers could distinguish the tolerant from the intolerant Sengon accessions. Therefore, SNP markers can be used to help the selection program for tolerant Sengon against Boktor pests.