Effect of storage time of pineapple peel waste on the characterization of bromelain produced for use in animal product processing

Abstract

This study examined the effect of pineapple stem storage time on the characteristics of the extracted bromelain and its potential antioxidant activity. Pineapple stems were stored at room temperature for a certain duration before bromelain extraction, with key parameters such as crude bromelain yield, enzyme activity, protein content, bromelain concentration, and antioxidant capacity analyzed. The results showed that crude bromelain yield increased with increasing storage time while enzyme activity, protein content, bromelain concentration, and antioxidant capacity decreased. These changes were likely influenced by physiological and biochemical processes in the pineapple stems during storage. Despite the decrease, bromelain and antioxidant capacity extracted from pineapple stems still showed significant potential as a bioactive source. This study highlights the importance of utilizing pineapple stems as a sustainable and value-added source of bromelain and antioxidants.