Standardization of Phomopsis sp. extract and prediction of anti breast cancer activity from the main compound through molecular docking

This title has been presented on Thursday, December 14, 2023 at 11.35-11.45 GMT+7.

Abstract

This title has been presented on Thursday, December 14, 2023 at 11.35-11.45 GMT+7.

The isolation of endophytic fungi from Indonesia soursop (Annona muricata) leaves, which have the potential to treat breast cancer, has been successfully carried out since 2016. Identification based on the Internal Transcribed Spacer (ITS) gene revealed that the fungi close to Phomopsis sp. The fungi have been stored, subcultured, and extracted with ethyl acetate for the in vitro and in vivo studies. To be registered as a medicinal ingredient and meet the requirements of the Indonesian Food and Drug Supervisory Agency (BPOM, Badan Pengawas Obat dan Makanan), it is necessary to ensure the standardization, safety, and quality of the Phomopsis extract. The results showed that Phomopsis extract met the BPOM requirements for organoleptic, water content, microbiological and heavy metal contamination, total aflatoxin content, weight uniformity, disintegration time, and solvent content. Analysis of the Phomopsis extract content also showed that the compounds in the two production batches were stable, with the greatest relative abundance is 7-hydroxycoumarin.